Lonza 96孔模塊優勢
1、高效轉染——采用上市20年的Lonza(原Amaxa)專利Nucleofector細胞核轉染技術,結合優化的電轉染程序、細胞特異性轉染試劑、無鋁離子毒害的新型聚合物電極,同時提高細胞轉染效率和轉染后細胞活率。
2、快速優化——真正的96個孔獨立轉染,可用于不同細胞轉染或同一細胞不同轉染條件的摸索,通過較低的細胞、轉染試劑、轉染耗材成本,短時間快速推進實驗進程。
3、兼容性——96孔模塊摸索的轉染條件,可方便地應用于4D核轉儀的X模塊和LV大體積轉染模塊、或Lonza 384孔高通量轉染系統,簡單實現轉染規模的線性放大。

4、自動化處理——96孔模塊可集成于液體處理工作站中,實現自動化處理,提升實驗效率,減少人工誤差。
參考文獻
1. Targeted gene knock-in by homology-directed genome editing using Cas9 ribonucleoprotein and AAV donor delivery. Thomas Gaj, Brett Staahl, Gonçalo Rodrigues, et al. Nucleic Acids Research, Volume 45, Issue 11, 20 June 2017, Page e98
2. Nucleofection-based gene targeting in human pre-B cells. Aya Kurosawa, Shinta Saito, Mikako Mori, Noritaka Adachi. Gene, Volume 492, Issue 1, 15 January 2012, Pages 305-308
3. Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation. Robert Bowles, Sonali Patil, Hanna Pincas, Stuart C. Sealfon. Immunology and Infection, July 8, 2011, doi: 10.3791/2766
4. An Engineered Zinc Finger Protein Activator of the Endogenous Glial Cell Line-Derived Neurotrophic Factor Gene Provides Functional Neuroprotection in a Rat Model of Parkinson's Disease. Josee Laganiere, Adrian Kells, Jeffrey Lai, et al. Neuroscience, 8 December 2010, 30 (49) 16469-16474
5. An RNA Interference Screen Reveals a Critical Role for Mcl-1 In Survival of CML Progenitor Cells. Su Chu and Ravi Bhatia. Blood, Volume 116, Issue 21, 19 November 2010, Page 1203
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——Lonza中國一級代理商,北京澤平
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